Flow cytometry immunophenotyping of peripheral blood circulating B-cell subset: comparison of osteoarthritis, chronic lymphocytic leukemia patients and normal donors

Prognostic importance of flow cytometric immunophenotyping of 540 consecutive patients with B-cell chronic lymphocytic leukemia.

Blood mononuclear cells from 540 newly diagnosed, unselected patients with B-cell chronic lymphocytic leukemia (CLL) were examined by immunofluorescence flow cytometry for a panel of surface membrane markers, including IgM and IgD, the monoclonal antibodies anti-CD3, -5, -20, -21, -22, -FMC7, and, for the final 125 patients, anti-CD23. There were 503 CD5+ and 37 CD5- cases. In the CD5+ cases, t...

Immunophenotyping of chronic B-cell neoplasms: flow cytometry versus immunohistochemistry

Morphological differentiation between benign and malignant lymphoproliferative disorders (LPDs) can be challenging. Immunophenotyping (IPT) by either technique, flow cytometry or immunohistochemistry (IHC), is an important step in solving such difficulty. Thirty-five newly diagnosed patients with chronic B-cell neoplasms (11 chronic lymphocytic leukemia, 22 non Hodgkin lymphoma and 2 hairy cell...

Bone marrow and peripheral blood hematopoietic reserve in patients with B-cell chronic lymphocytic leukemia.

haematologica/journal of hematology vol. 88(12):december 2003 1429 4. Soderholm J, Kobayashi H, Mathieu C, Rowley J, Nucifora G. The leukemia-associated gene MDS1/EVI1 is a new type of GATA-binding transactivator. Leukemia 1997; 11:352-8. 5. Horsman D, Gascoyne R, Barnett M. Acute leukemia with structural rearrangements of chromosome 3. Leuk Lymphoma 1995;16:369-77. 6. Reiter E, Greinix H, Rabi...

Do B-cell chronic lymphocytic leukemia patients with Ig VH3-21 genes constitute a new subset of chronic lymphocytic leukemia?

Normal cellular counterparts of B cell chronic lymphocytic leukemia.

In an attempt to compare B cell chronic lymphocytic leukemia (B-CLL) with its normal cellular counterpart, the cell surface phenotype of 100 cases of B-CLL was determined by using a panel of monoclonal antibodies (MoAbs) directed against B cell-restricted and -associated antigens. The majority of B-CLL cells expressed Ia, B4 (CD19), B1 (CD20), B2 (CD21), surface immunoglobulin (sIg), and T1 (CD...